|Title||HSP12 is essential for biofilm formation by a Sardinian wine strain of S. cerevisiae.|
|Publication Type||Journal Article|
|Year of Publication||2002|
|Authors||Zara, S, G Farris, A, Budroni, M, Bakalinsky, AT|
|Date Published||2002 Feb|
|Keywords||Alleles, Base Sequence, Biofilms, DNA Transposable Elements, DNA, Fungal, Heat-Shock Proteins, Molecular Sequence Data, Mutagenesis, Polymerase Chain Reaction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Nucleic Acid, Wine|
Sardinian sherry strains of S. cerevisiae form a biofilm on the surface of wine at the end of the ethanolic fermentation, when grape sugar is depleted and when further growth becomes dependent on access to oxygen. A point mutation in HSP12 or deletion of the entire gene results in inability to form this film. HSP12 encodes a heat-shock protein previously foundby others to be active during stationary phase, in cells depleted for glucose, and in cells metabolizing ethanol and fatty acids, all conditions associated with sherry biofilms.